The Expulsion from the Garden of Eden (Italian: Cacciata dei progenitori dall'Eden) is a fresco by the Italian Early Renaissance artist Masaccio.

Many possible sources of inspiration have been pointed out that Masaccio may have drawn from. For Adam, possible references include numerous sculptures of Marsyas (from Greek Mythology) and certain crucifix done by Donatello.

For Eve, art analysts usually point to different versions of Venus Pudica, such as Prudence by Giovanni Pisano.

Three centuries after the fresco was painted, Cosimo III de' Medici, in line with contemporary ideas of decorum, ordered that fig leaves be added to conceal the genitals of the figures. These were eventually removed in the 1980s when the painting was fully restored and cleaned.