The painting is relentlessly physical, from the wide spurts of blood to the energy of the two women as they perform the act. The effort of the women's struggle is most finely represented by the delicate face of the maid, who is younger than in most paintings, which is grasped by the oversized, muscular fist of Holofernes as he desperately struggles to survive. Although the painting depicts a classic scene from the Bible, Gentileschi drew herself as Judith and her mentor Agostino Tassi, who was tried for and convicted of her rape, as Holofernes. Gentileschi's biographer Mary Garrard famously proposed an autobiographical reading of the painting, stating that it functions as "a cathartic expression of the artist's private, and perhaps repressed, rage". She also painted a later version of the work in the early 1620s, now in the Uffizi in Florence.

Gentileschi's self-insertion was reversed in an influential composition by Cristofano Allori (c. 1613 onwards), which exists in several versions and copied a conceit of Caravaggio's recent David with the Head of Goliath; here the head is a portrait of the artist, Judith his ex-mistress, and the maid her mother.

Gentileschi painted another painting, Judith and her Maidservant (1613–14), which shows Judith holding a dagger while her maidservant carries a basket containing a severed head. Judith and her Maidservant is displayed in the Palazzo Pitti, in Florence. A further three paintings by Gentileschi, in Naples, Detroit and Cannes, show her maid covering the head of Holofernes, while Judith herself looks out the frame of the picture.

Judith beheading Holofernes has been depicted by a number of artists including Giorgione, Titian, Rembrandt, Peter Paul Rubens and Caravaggio.

Caravaggio's Judith Beheading Holofernes is believed to be the main source of this work, and his influence shows in the naturalism and violence Gentileschi brings to her canvas. In both there is a notable absence of decorative detail in the background. Gentileschi's father, Orazio Gentileschi, was also a famous painter; he was also very much influenced by Caravaggio's style and painted his own version of the tale, Judith and Her Maidservant with the Head of Holofernes.

According to wikipedia.